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Acyclovir release from mucoadhesive matrices
Šišáková, Lenka ; Šnejdrová, Eva (advisor) ; Dittrich, Milan (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Student: Lenka Šišáková Supervisor of Diploma thesis: PharmDr. Eva Šnejdrová, Ph.D. Title of Diploma thesis: Aciclovir release from mucoadhesive matrices The aim of this diploma thesis was the study of the mucoadhesive parameters of plasticized oligoester of lactic acid and glycolic acid and 3% mannitol as a branching monomer. Knowledge of dissolution testing of drug release from dosage form, principal theories and mechanisms of mucoadhesion and mucoadhesion testing of adhesive formulation is described in theoretical part. Matrices formed from terpolymer of D,L-lactic acid, glycolic acid branched with mannitol and 5 % aciclovir were examined in the experimental part. Triethylcitrate (TEC), ethylpyruvate (EP), methylsalicylate (MS) and ethylsalicylate (ES) were used as plasticizers. Dissolution test has been done. Hydrated mucin from porcine stomach was used as a base. Phosphate-citrate buffer pH 7.4 was used as a dissolution medium. Dissolution was defined as a quantity of released aciclovir in to the dissolution medium after 15, 30, 60 and 90 minutes. The quantity of the released aciclovir was defined by a spectrophotometry. In 90 minutes was released 43 % of aciclovir from the matrice...
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Polymer systems for siRNA delivery
Blažková, Jana ; Pechar, Michal (advisor) ; Šťovíček, Vratislav (referee)
The process of RNA interference (RNAi) is a natural phenomenon posttranscriptionally controlling gene expression by means of small double-stranded RNA molecules (dsRNA). Small interfering RNA (siRNA) is a small dsRNA that can be used for targeted gene silencing as an alternative therapeutic treatment of genetic diseases. For in vivo administration, siRNA must be protected against degradation to ensure its efficient delivery to target cells using sophisticated vectors. This work is focused on description of non-viral vectors based on cationic polymers, forming polyelectrolyte complexes with siRNA (polyplexes), and surface-modifying hydrophilic polymers enabling protection of the vector during its transport in the bloodstream.
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Acyclovir release from mucoadhesive matrices
Šišáková, Lenka ; Šnejdrová, Eva (advisor) ; Dittrich, Milan (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Student: Lenka Šišáková Supervisor of Diploma thesis: PharmDr. Eva Šnejdrová, Ph.D. Title of Diploma thesis: Aciclovir release from mucoadhesive matrices The aim of this diploma thesis was the study of the mucoadhesive parameters of plasticized oligoester of lactic acid and glycolic acid and 3% mannitol as a branching monomer. Knowledge of dissolution testing of drug release from dosage form, principal theories and mechanisms of mucoadhesion and mucoadhesion testing of adhesive formulation is described in theoretical part. Matrices formed from terpolymer of D,L-lactic acid, glycolic acid branched with mannitol and 5 % aciclovir were examined in the experimental part. Triethylcitrate (TEC), ethylpyruvate (EP), methylsalicylate (MS) and ethylsalicylate (ES) were used as plasticizers. Dissolution test has been done. Hydrated mucin from porcine stomach was used as a base. Phosphate-citrate buffer pH 7.4 was used as a dissolution medium. Dissolution was defined as a quantity of released aciclovir in to the dissolution medium after 15, 30, 60 and 90 minutes. The quantity of the released aciclovir was defined by a spectrophotometry. In 90 minutes was released 43 % of aciclovir from the matrice...
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Effect of plasticization on rheological properties of oligoester of lactic acid and glycolic acid branched with dipentaerythritol
Bílková, Klára ; Šnejdrová, Eva (advisor) ; Dittrich, Milan (referee)
CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical technology Name of the student: Klára Bílková Title of diploma thesis: Influence of plastification on rheological properties of oligoester of lactic acid and glycolic acid branched with dipentaerythritole Consultant: PharmDr. Eva Šnejdrová, Ph.D. The aim of this diploma thesis was the study of rheological properties of the oligoester of DL-lactic and glycolic acids branched with 1% of dipenthaerythritol (1D) and plasticized with 6 various plasticizers in increasing concentrations. Theoretical part was devoted to fundamentals of rheology and measurements of viscosity using rotational viscometers. It describes basic types and constructions of rotational rheometers and summarizes basic facts about bioadhesion and use of rheological method for assessment of bioadhesion. There were prepared matrices from oligoester 1D and plasticizers in concentrations of 20 %, 30 % and 40 % in the experimental part. These plasticizers were tested: ethyl pyruvate, ethyl salicylate, methyl salicylate, triacetin, tributyrin and triethyl citrate. Rheological properties were examinated at 37 řC using spindle viscometer and at 37 řC and 50 řC using rotational rheometer. Rheograms were used to characterize flow properties of tested...
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Polymer systems for siRNA delivery
Blažková, Jana ; Pechar, Michal (advisor) ; Šťovíček, Vratislav (referee)
The process of RNA interference (RNAi) is a natural phenomenon posttranscriptionally controlling gene expression by means of small double-stranded RNA molecules (dsRNA). Small interfering RNA (siRNA) is a small dsRNA that can be used for targeted gene silencing as an alternative therapeutic treatment of genetic diseases. For in vivo administration, siRNA must be protected against degradation to ensure its efficient delivery to target cells using sophisticated vectors. This work is focused on description of non-viral vectors based on cationic polymers, forming polyelectrolyte complexes with siRNA (polyplexes), and surface-modifying hydrophilic polymers enabling protection of the vector during its transport in the bloodstream.
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